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Fig. 6. 14-3-3{sigma} was markedly increased in the cytoplasm of suprabasal K10-/- keratinocytes (B) compared with the wild-type (A). (C) Protein extracts of skin revealed a strong increase in 14-3-3{sigma} expression in K10-/- mice (lane 5) compared with the wild-type (lane 4; lane 1, marker; lanes 2,3, Coomassie-stained blot of lanes 4,5; dots from top to bottom: 36 kDa and 26 kDa). (D) Northern analysis showed an equally strong increase in 14-3-3{sigma} mRNA (lane 4) over the wild-type (lane 3; lanes 1,2, ethidium-stained agarose gel corresponding to lanes 3,4; dots from top to bottom: 2.8, 1.8 and 1.5 kb). Bar, 40 µm.