Fig. 2. Effects of PEX2 depletion on matrix protein import and glycosome morphology in bloodstream form trypanosomes. Cells were grown without Tet (control, -Tet) or in the presence of 100 ng/ml Tet for 24 hours (+Tet). For the top and middle rows, ALD was stained with AlexaFluor 488 and PEX11 was detected by a Cy3-coupled secondary antibody. In the bottom row, PEX11 was Alexa-stained and HXK detected by Cy3. Corresponding Nomarski images and DAPI staining are shown on the left. Bar, 10 µm.