Fig. 1. Morphologies of BVMs cultured with or without MK. BVMs were cultured in DMEM-FCS for 1 day (24 hours) (A,C,E,G) or 3 days (72 hours) (B,D,F,H) with (C-H) or without (A,B) 100 ng/ml of MK. For antibody inhibition studies, anti-MK antibody (90 µg/ml) (E,F) or anti-IL-8 antibody (30 µg/ml) (G,H) was added. Then, BVMs were embedded in paraffin, cut into 5 µm sections and stained with hematoxylin-eosin. (A,C) HUVECs were attached and formed a monolayer on the HASMC-populated collagen gel on Day 1. (B) HUVECs were attached and formed a monolayer and secreted extracellular matrix on the HASMC-populated collagen gel on Day 3. (D) HUVECs were stratificated on the HASMC-populated collagen gel on Day 3. The extracellular matrix was secreted in large quantities. Bar, 100 µm.