Fig. 2. Anti-PCNA immunostaining of BVMs. (A,B,E,F) Control staining without the first antibody on Day 1 (A,E) and on Day 3 (B,F). (C,D,G,H) Anti-PCNA immunostaining on Day 1 (C,G) and Day 3 (D,H). BVMS were either untreated (A,B,C,D) or cultured with 100 ng/ml of MK (E,F,G,H) as in Fig. 1.