Fig. 6. Effects of treatment with 100 ng/ml MK for 24 hours on expression of PTP, LRP and PG-M/versican. (A) Proteins were separated by SDS-PAGE, and PTP and LRP were detected by western blotting with the respective antibody. The intensity of bands was normalized to that of actin, and each band was shown as the ratio of the value in the treated cells to that in the non-treated cells. (B) Expression of versican revealed by RT-PCR. The intensity of bands was normalized to that of GAPDH and was shown as the ratio of the value in the treated cells to that in the non-treated cells.