Fig. 2. Tyrosine phosphorylation of focal contact proteins. The GD25ß1A and GD25ß1B cell lines were allowed to attach to invasin for 60 minutes and then lysed in RIPA buffer. The lysates were immunoprecipitated for FAK, p130Cas or paxillin, and the precipitated proteins were analyzed by western blotting using antibodies specific for phosphotyrosine. As a loading control, the membranes were stripped and incubated with protein-specific antibodies.