Fig. 5. Distribution of the different ß1-integrin variants in cells grown in culture. The indicated cells were seeded on glass cover slips and incubated overnight in culture medium after which they were fixed and stained by anti-ß1 -integrin antibodies followed by fluorescein-conjugated secondary antibodies. The specimens were analyzed using a fluorescence microscope, and images were taken using a CCD camera. Bar, 10 µm.