Fig. 6. Distribution of the different ß1-integrin variants in cells adhering to invasin. GD25 cells expressing different variants of the ß1-integrin were allowed to spread on GST-invasin-coated glass cover slips for 3 hours. The cells suspended in serum-free DMEM were pretreated with cycloheximide (25 µg/ml) and GRGDS (0.1 mg/ml). After fixation, the cells were double-stained with anti-ß1-integrin (Aa-Ka) and anti-vinculin (Ab-Kb) antibodies or with anti-ß1-integrin (Ac-Kc) and anti-phosphotyrosine (Ad-Kd) antibodies. The specimens were analyzed using a fluorescence microscope and images were taken using a CCD camera. Bar, 10 µm.