Fig. 1. Fak^-/- MEFs are deficient for Y. pseudotuberculosis uptake. (A) The percentage of internalized Y. pseudotuberculosis observed in infected Fak^+/+, Fak^-/- and Fak^-/- MEFs expressing ectopic Fak is presented as the average of eight experiments. Error bars indicate standard deviation from the mean. (B) A representative immunoblot showing Fak expression from lysates derived from Fak^+/+, Fak^-/-, and Fak^-/- MEFs reconstituted to express Fak. (C) Overlay of fluorescence images (400x magnification) of infected Fak^-/- MEFs, including one Fak-transfected cell (blue). Intracellular Y. pseudotuberculosis appear red and extracellular Y. pseudotuberculosis appear yellow.