Fig. 3. Cas promotes Fak-independent Y. pseudotuberculosis uptake. (A) Fak^-/- MEFs were transfected with the designated amounts of a plasmid encoding Cas. Uptake levels for each condition were compared by ANOVA to the level observed for mock-transfected cells. The data presented were obtained from 11 independent experiments. Uptake of YP17/pVector into mock-transfected cells averaged 35%. Error bars represent limits at a 95% confidence level. (B) Representative Cas immunoblot showing Cas expression in lysates from Fak^-/- MEFs transfected with the designated amounts of DNA Cas.