Fig. 5. LIX and TTP are induced during C2C12 myoblast activation in culture. RNA isolated from a time course of cell cycle activation was probed with each fragment described in Fig. 4. Mb, asynchronous myoblasts; S12, S48, S60, cells held in suspension for 12, 48 or 60 hours, respectively; R 0.5-30, cells replated for 0.5 to 30 hours after arrest in suspension; Mt, myotubes. All four transcripts are detected in G0 synchronized cells and further induced transiently in newly activated cells. Data are representative of three independent experiments.