Fig. 7. LIX and TTP are rapidly induced in response to muscle injury. RNA was isolated from uninjured adult mouse skeletal muscle (0) and at different times post injury (PI) and probed for TTP and LIX expression. LIX is induced at 6 hours and peaks prior to the peak of proliferation (assessed by Histone H2B mRNA). TTP was activated by 30 minutes after injury, well before the activation of MyoD. Each lane represents pooled RNA (20 µg) from TA muscles of two to three mice. Data are representative of three independent experiments.