Fig. 8. LIX and TTP transcripts are distributed in a pattern similar to MyoD in injured skeletal muscle. RNA in situ hybridization to frozen sections (20 µm) of injured TA muscle using digoxigenin-labeled antisense probes to LIX, MyoD and TTP transcripts (A, B and C, respectively). Absence of staining in the myofiber interior suggests sequestration of the transcripts in mononuclear cells at the myofiber periphery. The same probes show no hybridization to uninjured muscle (a, b and c). LIX and MyoD were detected 6 hours post injury and TTP at 3 hours post injury. (D) shows TTP-positive cells located in the lesion; an adjacent uninjured area is devoid of TTP transcripts. Data are representative of three independent experiments each involving multiple cryosections from two mice.