Fig. 1. CD44 and ezrin localisation in A431 cells. (A) A431 cells were serum starved for 2 days then processed for immunofluorescence using the E1/2 CD44 monoclonal antibody (a) and an anti-ezrin polyclonal antibody (b) (arrow indicates CD44-positive basal plaque; see text). (B) Serum-starved A431 cells were treated with 10 ng/ml EGF for 5 minutes before processing for immunofluorescence using the E1/2 CD44 antibody (a, b) or the ezrin antibody (c, d). Images (a, c and e) are confocal sections chosen to highlight membrane ruffling (open arrow), whereas images (b, d and f) are a higher confocal section showing microvilli (filled arrow). Panels e and f represent merged images (yellow) of a, c and b, d respectively. Bar, 10 µm.