Fig. 2. CD44 localisation and expression in A431 cells expressing dominant-negative Jun. (A) NA and TA cells were serum starved for 2 days (a, c) or treated with EGF for 5 minutes (b, d), then processed for immunofluorescence using the E1/2 CD44 monoclonal antibody. Bar, 10 µm. (B) Whole cell extracts from serum-starved and EGF-treated NA and TA cells were prepared and analysed by western blotting using the E1/2 CD44 monoclonal antibody. CD44s is the standard 85 kDa isoform; CD44E indicates the 145 kDa, epithelial isoform.