Fig. 3. Ezrin localisation and expression in A431 cells expressing TAM67. (A) NA and TA cells were serum starved for 2 days followed by treatment with 10 ng/ml EGF for 5 minutes. Cells were fixed then immunostained for ezrin distribution and counter-stained with phalloidin to visualise actin organization. Bar, 10 µm. (B) Whole cell extracts from serum-starved and EGF-treated NA and TA cells were prepared and analysed by western blotting using an ezrin polyclonal antibody.