Fig. 4. Effect of Protein Kinase C inhibition on CD44 and ezrin localisation in A431 cells. NA cells were serum starved for 2 days then pretreated with or without 3 µM Ro-31-8220 for 30 minutes, followed by treatment with 10 ng/ml EGF for 5 minutes. Cells were fixed then immunostained for either CD44 (A) using the E1/2 monoclonal antibody and counterstaining with FITC-conjugated phalloidin or ezrin (B) using a polyclonal ezrin antibody and counterstaining with TRITC-conjugated phalloidin. Bar, 10 µm.