Fig. 5. CD44 and ezrin localisation after phorbol ester treatment of NA and TA cells. NA cells (A, C) and TA cells (B, D) were serum starved for 2 days then treated with 100 ng/ml TPA for 5 minutes. After fixation, cells were immunostained for CD44 using the E1/2 monoclonal antibody (A, B, lower panels) or for ezrin using a polyclonal ezrin antibody (C, D). Cells were counterstained with phalloidin (A, B, upper panels) to visualise actin redistribution to cell-cell contacts (arrowheads). Bar, 10 µm.