Fig. 6. Protein Kinase C isoform expression in NA and TA cells. (A) NA and TA cells were serum starved for 2 days then left untreated or treated with 10 ng/ml EGF for 5 minutes. Whole cell extracts were prepared and analysed by western blotting using antibodies for PKC, PKC, PKC and PKC. (B) Total RNA was prepared from NA and TA cells then 20 µg samples were electrophoresed, blotted then probed with a radiolabelled PCR fragment of human PKC. Equivalent loading of RNA samples was demonstrated using a 7S ribosomal cDNA probe.