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Fig. 5. Trafficking of M-C6-NBD-PC to the vacuole requires vesicular transport from the PVC to the vacuole. (A) The sec12-4 mutant strain and its wild-type parent were labeled as described in Fig. 2. (B,C) The wild-type and deletion strains were grown to early log phase in SDC at 30°C and labeled as described in Fig. 1.