Fig. 5. Activation of the RhoA pathway by LPA and serum stimulation. Immunofluorescence images show SW13 cells starved (0.25% fetal calf serum) for 6 hours (A) and exposed to 10 µM LPA after 6 hours of starvation (B) before being fixed and stained with FITC-phalloidin. Immunofluorescence images show BRG1-expressing cells starved (0.25% fetal calf serum) for 18 hours (C), exposed to 5 µM LPA for 20 minutes after starvation (D) and given 5% serum for 20 minutes after starvation (E). Immunofluorescence images show BRG1-expressing cells expressing a myc-tagged RhoA(N19) protein stained with FITC-phalloidin (F) and the anti-9E-myc antibody (G) for detection of cells expressing the RhoA(N19) protein. All images are at the same magnification; bar, 20 µm.