Fig. 8. The effect of Rho-kinase/ROCK expression in SW13 cells. (A,B) Immunofluoroscence images show SW13 cells expressing a myc-tagged, constitutively active Rho-kinase/ROCK fragment habouring the kinase domain, which were fixed and stained with FITC-phalloidin (A) and the anti-9E-myc antibody (B) for detection of cells expressing the Rho-kinase/ROCK fragment. Immunofluorescence images of cells expressing a wild-type, myc-tagged ROCK1 protein stained with FITC-phalloidin (C) and anti-9E-myc antibodies (D), and SW13 cells expressing a wild-type, myc-tagged ROCK2 protein stained with FITC-phalloidin (E) and anti-myc-9E-antibodies (F). Immuofluorescence images of BRG1-expressing cells transfected with a dominant negative, myc-tagged ROCK1 (KD-IA), stained with FITC-phalloidin (G) and anti-9E-myc antibodies (H). Bar, 20 µm; the same magnification was used in A-H.