Fig. 4. Histology of SPARC-null lens at 3 months of age. Sections of wt and SPARC-null lenses were stained with hematoxylin and eosin. Loss of normal shape of secondary fiber cells in SPARC-null lenses is shown. Comparable center cortical anterior regions from ^+/+ (A,C,E,G) and SPARC-null (^-/-) lenses (B,D,F,H) are shown. Note that in the SPARC-null lens, most of the secondary fiber cells have become rounded and swollen, and the nucleus has been displaced toward the posterior capsule (B). Bar (A,B), 540 µm; bar (C-H), 18 µm.