Fig. 8. Recruitment of cIAP1 and cIAP2 into the TNF-R2 complex critically depends on TRAF2 in living cells. HeLa-TNF-R2 cells were transfected with expression plasmids encoding for cIAP1(NT)-GFP (A) or cIAP1(NT)-GFP (B), alone or in combination with an expression construct for full-length TRAF2. The following day, distribution of the GFP chimeras was both analyzed prior to stimulation (2 µg/ml TNF-R2 IgG) and monitored online for 15 minutes after stimulation using confocal microscopy. Examples of ligand-induced alterations in the localization of the GFP fusion proteins are indicated with arrows.