Fig. 3. ß-catenin phosphorylated on serines 33 and 37 can undergo dephosphorylation. (A) GFP-ß-catenin was transfected into 293T cells, and after 24 hours the cells were treated with either MG132 (lanes 1-4), MG132 plus LiCl (lanes 5-8) or treated with MG132 and LiCl and then washed and incubated with MG132 only (lanes 9-12). (B) 293T cells were treated with MG132 (lanes 2-5), left untreated (lane 1) or incubated with MG132 and LiCl (lanes 6-9) or treated with MG132 and LiCl and then washed from the LiCl and left with only MG132 (lanes 10-12). Cell extracts in (A) and (B) were analyzed by western blotting with pß-catenin antibody and reprobed with general anti-ß-catenin antibody. (C) Quantitative analysis of the level of phosphorylated ß-catenin is shown in B. The numbers correspond to intensities of the signal with pß-catenin antibody normalized to the signal obtained with the general anti-ß-catenin antibody.