Fig. 4. pß-catenin can form a complex with LEF-1 but does not form a ternary complex with LEF-1 bound to DNA. (A) VSV-ß-catenin was co-transfected with HA- LEF-1 into 293T cells and immunoprecipitated with anti HA antibody as a complex with LEF-1. Cell extracts were analyzed by western blotting with the pß-catenin antibody. The asterisk marks the Ig heavy chain of the antibody. (B) VSV-ß-catenin was transfected into 293T cells and immunoprecipitated with ß-catenin or with pß-catenin antibodies as indicated. (C) ß-catenin—LEF-1—DNA complex formation was analyzed with 6 µg of nuclear extracts from 293T cells transfected with LEF-1 and VSV-ß-catenin that were incubated with ³²P-labeled DNA containing the LEF-1-binding site, in the absence or presence of the indicated antibodies. Protein-DNA complexes were separated by native 4% PAGE and visualized by autoradiography. The asterisk marks a non-specific band. IP, immunoprecipitation; IB, immunoblot.