Fig. 5. pß-catenin is hardly ever found in complex with N-cadherin. 293T cells were transfected with ß-catenin together with either N-cadherin or with F-ß-TrCP, which served as a control for complexing with pß-catenin. Cell extracts were immunoprecipitated as indicated and analyzed by western blotting with both the pß-catenin and a general anti-ß-catenin antibodies. The asterisks mark the Ig heavy chain of the antibody.