Fig. 6. Phosphorylated p38 accumulates in the nucleus in both adherent and non-adherent cells. Serum-starved detached NIH 3T3 cells were either plated onto fibronectin (Fn)-coated dishes or maintained in suspension (Sus) for 2 hours in DMEM/BSA before being stimulated with 50 ng/ml anisomycin for 30 minutes. Suspended cells were allowed to adhere to poly-lysine-coated coverslips for the final 15 minutes. Fixed cells were analyzed by immunofluorescence for phospho-p38. Bars, 20 µM. Inserts show the overlay between phospho-p38 and nuclear staining with Hoechst 33342 reagent.