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Fig. 3. Colchicine-treated meristematic metaphase cells of rye. (a) Simultaneous visualization of chromatin (DAPI staining in blue) and of the rDNA hybridization sites (red) reveals the presence of two NORs exhibiting a centromere-proximal block of rDNA followed by a ribosomal chromatin filament towards the satellite (brackets). Note the clear length differences between the decondensed regions of the two homologous rDNA loci. (b) The simultaneous visualization of the in situ hybridization and the silver staining signals shows that the Ag-positive rDNA fraction is found only coincident with the distended ribosomal chromatin revealed by FISH (compare with a). Bar, 5 µm.