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Fig. 7. Simultaneous visualization of chromatin (DAPI staining in blue) and rDNA hybridization sites (red) in meristematic interphase nuclei of rye illustrating the relative positioning rDNA loci. (a) Adjacent rDNA loci. (b) Fused rDNA loci (hybrid rye line). Bar, 5 µm. Note that rDNA perinucleolar knobs are usually present on the nuclear hemisphere where DAPI staining is more intense, indicating a higher concentration of chromatin in this pole of the nucleus.