Fig. 3. An analysis of the behaviour of Cdc16^RNAi and Cdc27^RNAi cells. (A) A western blot showing the levels of Cdc16 (panel 1) and Cdc27 (panel 3) in mock-treated (-) or RNAi-treated (+) cells. These blots were also probed with an anti-tubulin antibody as a loading control (panels 2,4). (B) A FACs analysis of cells at various time points after RNAi treatment. (C) A graph showing the total viable cell numbers in control and RNAi-treated cells at various time points after RNAi treatment. Results were pooled from four independent experiments and error bars indicate the standard deviation. (D) A graph showing the percentage of TUNEL-positive cells after the different RNAi treatments (as indicated under each bar). Only data from the 72 hour time point is shown. Error bars represent the standard deviation.