Fig. 7. An analysis of the levels of various cell cycle proteins in
Cdc16RNAi and Cdc27RNAi cells. The western blots show
the levels of Cdc16 (panel 1), Cdc27 (panel 2), Cyclin A (panel 3), Cyclin B
(panel 4) and Fzy (panel 5) in mock-treated (-) or RNAi-treated (+) cells. All
blots were also probed with anti-tubulin antibodies to confirm an equal
loading (data not shown), and we show the levels of a non-specific band that
is variably recognised by the anti-Cdc16 antibodies (asterisk, panel 1). A
slower migrating form of Cyclin A is highlighted with an arrowhead in the
Cdc27RNAi- and Cdc16RNAi- and
Cdc27RNAi-treated cells.