Fig. 6. Lack of a dominant-negative effect of PKB[KD]GLUT4 on insulin-stimulated Forkhead translocation out of the nucleus of 3T3-L1 adipocytes. 3T3-L1 adipocytes were microinjected with a plasmid encoding a GFP-tagged human FKHR in the absence (A) or presence (B) of the PKB[KD]-GLUT4 plasmid. After 24 hours the cells were serum starved and treated without (left-hand panels) or with (right-hand panels) insulin for 30 minutes as indicated. The cells were fixed and examined for the distribution of the FKHR-GFP by confocal microscopy. In C, the amount of nuclear FKHR was quantified as described in the Materials and Methods. The results shown represent means±s.d. for at least three independent experiments with each condition represented by a minimum of 11 cells. ^*P<0.001 versus control incubated without insulin and no significant difference from the insulin-stimulated cells expressing PKB[KD]-GLUT4. ^#No significant difference from control in the absence of PKB[KD]-GLUT4.