Fig. 8. Localization of EGFP-EFA6A-PHCT1 (A), PHCT2 (B), PHCT3 (C) and PHCT4 (D) in BHK cells. (These constructs, which include the PH domain, start at position 349 and their C-terminus is indicated, see also Fig. 9C). Expression of PHCT1-3 constructs induced the formation of finger-like protrusions on the dorsal cell surface. By contrast, deletion of the conserved C-terminal coiled-coil motif in PHCT4 prevented the induction of these structures, and this construct accumulated in short microvilli-like protrusions. Confocal sections were taken in the dorsal plane of the cells. Bar, 10 µm.