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Fig. 2. H1.X detection in the marginal cells of the C. elegans pharynx. (A) represents an antibody labeling performed with {alpha}H1.X-11; (B) represents the corresponding Nomarski micrograph. (C) represents an antibody labeling performed with {alpha}H1.X-101. (D) shows H1.X::GFP expression in the marginal cells in a view lateral to the flat cell bodies. Here the tonofilaments are viewed in lateral orientation. (E) is the corresponding Nomarski micrograph. (F) shows H1.X::GFP expression in the marginal cells in an optical cross-section of the flat cell bodies. Here the tonofilaments appear in axial orientation as bright green fluorescent dots. H1.X::GFP shows a cytoplasmic as well as a nuclear localization (arrows point to the nuclei) of the fusion protein. Bar, 20 µm.