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Fig. 6. Nucleolar localization of H1.X (A) shows a Nomarski micrograph of the nuclear region of a polyploid gut cell in a hermaphrodite C. elegans with the nucleolus and its substructures visualized by differential interference contrast. (B) H1.X detected with the antibody {alpha}H1.X-101 localizes to the nucleolus; (C) corresponds to DAPI DNA staining; and (D) shows the indirect immunfluorescence detection of the nucleolar protein fibrillarin with the specific monoclonal antibody P2G3 (from M. Christensen). Bar, 10 µm.