Fig. 8. Expression of H1.X::GFP in living (A) and in formaldehyde-fixed HeLa cells (C). The nucleoli are prominently fluorescent in the fixed and extracted cells, whereas in living cells the whole nucleoplasm fluoresces. The brightest spots in the nucleoplasm of the living cells correspond to the nucleoli. (B,D) represent the corresponding Nomarski micrographs. Bars, 10 µm.