Fig. 8. Digestion of a yeast particle indicated by Neutral Red staining of the yeast cytoplasm. Neutral Red is excluded from living yeast particles but floods and stains the cytoplasm of phagocytosed yeast particles when digestion has progressed sufficiently. This transition takes place suddenly. VatM-GFP is still present in the phagosome membrane at this time. The image in B was recorded 30 seconds after the image in A. Bar, 10 µm.