Fig. 1. Mutant opsin accumulates in a structure close to the Golgi apparatus. The cellular localisations of opsin in transiently transfected COS-7 cells expressing WT opsin and WT opsin with a C-terminal GFP tag (WT-GFP) were compared with mutant opsin (P23H-opsin) and GFP-tagged mutant opsin (P23H-GFP) by confocal immunofluorescence. The intracellular opsin colocalised with the Golgi marker ß-COP; however, in cells with mutant opsin inclusions, the ß-COP staining is dispersed. Bar, 10 µm.