Fig. 3. Mutant opsin inclusions have the characteristics of an aggresome. Mutant opsin (P23H-GFP) colocalised with c-myctagged ubiquitin when co-transfected in COS-7 cells. The P23H-GFP opsin inclusions led to the disruption of the intermediate filament network (Vimentin). The cytoplasmic chaperone Hsc70 was recruited to the P23H-GFP inclusions. Bar, 10 µm.