Fig. 3. Localization of `activated' <sub>v</sub>ß<sub>3</sub> integrin in live and fixed cells. Human OCs were stained before (A-C) or after (D-F) fixation with the monoclonal activating Ab WOW-1 (red), which binds to the `activated' form of _vß₃, and costains with fluorescein-tagged phalloidin to detect the actin organization (green). The `activated' form of <sub>v</sub>ß<sub>3</sub> (A,D, arrows) is specifically organized at the cell edge where it colocalized with cortical actin (B,E, arrows). Clusters of podosomes are also evident in both fixed and live cells, but in both cases the `activated' form of _vß₃ is not organized around the podosomal core of actin filaments as shown in the overlay (C,F, asterisk)