Fig. 2. RNA in situ hybridization analysis of AKAP95 message in MII oocytes. Oocytes were fixed and hybridized with a biotinylated probe against rat AKAP95 cDNA. Hybridization was detected with TRITC-conjugated avidin (red). DNA was counterstained with 0.2 µg/ml Hoechst 33342 (blue). Samples were also pre-treated with DNAse I (+DNAse) or 100 µg/ml RNAse A (+RNAse) prior to fixation. Note the restricted localization of AKAP95 mRNA around the meiotic spindle. PB, polar body. Dotted circles delineate the oocyte. Bar, 20 µm.