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Fig. 3. Distribution of mCAP-D2 in mouse gametes. (A) Immunoblotting analysis of mCAP-D2 in indicated cell types using an affinity-purified antibody against hCAP-D2. (B) Immunofluorescence localization of mCAP-D2 in MII oocytes. Arrowhead points to MII chromosomes. DNA was stained with Hoechst 33342. PB, polar body. Bar, 20 µm.