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Fig. 5. AKAP95 is required for condensation of maternal chromosomes. (A) Zygotes produced by ICSI of Hoechst-labeled sperm were injected into the FPN or the MPN with 250 pg AKAP95(387-450). Embryos were fixed, counterstained with PI (red) and labeled using anti-AKAP95 polyclonal antibodies (green). (B-D) Injected embryos were cultured to mitosis, fixed and analyzed by DNA staining and AKAP95 immunofluorescence using the polyclonal antibody or mAb47 (insets in B and C only). (B) Mitosis after MPN injection. (C) Mitosis after FPN injection. (D) Mitosis after FPN injection with 250 pg AKAP95(387-692). `F' and `M', female and male chromatin, respectively; PB, polar body. Dotted circles delineate embryos. Bar, 20 µm.