Fig. 6. AKAP95 is required for mCAP-D2 targeting to maternal, but not paternal, chromatin. (A,B) Injection of AKAP95(387-450) in the FPN abolishes mCAP-D2 targeting to female chromatin. The FPN of zygotes produced as in Fig. 5A was injected with (A) 250 pg AKAP95(387-450) or (B) 250 pg AKAP95(387-692). Embryos progressed to mitosis and were examined by total DNA staining with PI (red) and mCAP-D2 immunofluorescence (green). (C,D) AKAP95(387-692) rescues condensation of maternal chromosomes. Mitotic embryos with decondensed female chromatin and condensed male chromosomes were cytoplasmically injected with (C) 5 ng AKAP95(387-692) or (D) 5 ng AKAP95(387-450) as a negative control. Embryos were fixed 2 hours later and analyzed by DNA labeling with PI and by immunofluorescence using the anti-AKAP95 polyclonal antibody. Arrowheads point to female chromatin. Color coding for C and D is shown. `F' and `M', female and male chromatin, respectively. Dotted circles delineate the embryos. Bar, 20 µm.