Fig. 8. AKAP95 is required for cleavage beyond the two-cell stage. The nucleus of one blastomere of a two-cell stage embryo was injected with 250 pg AKAP95(387-450) in a buffer containing a 150 kDa FITC-dextran as a tracer (arrow), while the second blastomere was injected with unlabeled peptide buffer. Each nucleus of control two-cell stage embryos was also mock-injected with buffer (Mock-injected, right panel). Embryos were allowed to cleave to the 8-16-cell stage. Nuclei were labeled with Hoechst 33342. Arrowheads point to a metaphase plate in the cleaving buffer-injected blastomere (Mitosis). PB, polar body. Bar, 20 µm.