Fig. 3. Effect of BzlGalNAc on incorporation of [³⁵S]sulfate, [³H]glucosamine and [³H]leucine into acinar cell proteins. (A) Equal aliquots of acini (0.4 mg protein) were incubated with the indicated concentrations of BzlGalNAc. 10% of the cell pellets were run on 7.5% gels and imaged on a phosphor screen for [³⁵S]sulfate. (B) 12.5% gel using 10% of the same cell pellets shown in A. Note the labeled diffuse area representing sulfated BzlGalNAc derivatives. (C) Quantitation of sulfate labeling versus [BzlGalNAc]. Muclin and p82/p75 were quantified from 7.5% gels, and prolipase and proelastase IV were quantified from 12.5% gels. (D) [³H]glucosamine labeling of equal aliquots (1 mg protein) of isolated acini in the presence of BzlGalNAc; 10% of the cell pellets were separated by SDS-PAGE followed by autoradiography. (E) [³H]leucine labeling of isolated acini in the presence of BzlGalNAc. Data are means±s.d. of triplicate samples from representative experiments.