Fig. 6. Assessment of cell viability after incubation with NaCl, chlorate, DMSO or BzlGalNAc. Acini were incubated for the indicated times and then labeled for 10 minutes with calcein-acetoxymethyl ester and ethidium homodimer to label live and dead cells, respectively. (A-H) The cells were imaged by fluorescence microscopy and representative images are shown. Live cells accumulate calcein and fluoresce green (E,G,H). Dead cells are stained by ethidium homodimer and fluoresce red (A-D). Triton X-100 (1%) was used to kill the cells which cannot accumulate calcein (F) but admit ethidium homodimer, which fluorescently stains nuclei red (B). (I) Scion Image analysis software was used to quantify the amount of red staining ethidium homodimer under the different conditions. The amount of dead cells in each sample was calculated as the percentage of ethidium homodimer labeled nuclei relative to Triton X-100 killed cells.