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Fig. 8. Effects of chlorate and BzlGalNAc on immunolocalization of Muclin and amylase after in vitro zymogen granule repletion. Pancreatic lobules were prepared from zymogen-granule-depleted mice and incubated in vitro for 4 hours in buffer supplemented with (A) 30 mM NaCl, (B) 30 mM chlorate, (C) 2% DMSO or (D) 32 mM BzlGalNAc. Cryosections were prepared and dual immunostained for Muclin (green) and amylase (red) and for DNA with DAPI (blue). Note larger Muclin- and amylase-positive structures in chlorate-treated tissue (arrows in B) compared with controls; and dramatic dissociation of Muclin (arrows in D) and amylase (arrowheads in D) in BzlGalNAc-treated tissue.