Fig. 6. Relocation of syntaxin 3 in response to stimulation of minor regulated pathway. Parotid tissue was processed for immunofluorescence following a 3 minute treatment with 40 nM CCh or 1 µM Iso. (A,B,D) Immunofluorescent specimens were stained with syntaxin 3 (red) and counterstained with BODIPY-phallacidin (green) or DPPIV (green). (C) Comparison of staining of DPPIV (red) and BODIPY-phallacidin (green). Open arrowheads identify examples of syntaxin 3 foci that have relocated to the luminal side of the actin band or into the DPPIV band. Bar, 10 µm.